File tree 1 file changed +18
-5
lines changed
1 file changed +18
-5
lines changed Original file line number Diff line number Diff line change 1
- Describe how to configure the workflow (using config.yaml and maybe additional files).
2
- All of them need to be present with example entries inside of the config folder.
3
-
4
- In config/config.yml:
5
- * Add the name of the folder containing the bamfiles under data/folder
1
+ The starting point of the pipeline are .bam files from one or more RNA samples. They are assumed to have been already sequenced.
2
+ Edit the config/config.yml file as follows:
3
+ * data:
4
+ 1 ) [ "folder"] : path to the folder containing the .bam files
5
+ * reference:
6
+ 1 ) [ "folder"] : the folder containing the .fa file
7
+ 2 ) [ "genome"] : the genome file
8
+ * known_sites: used only in FreeBayes, and not GATK's HaplotypeCaller. Currently not used
9
+ * filtering:
10
+ 1 ) [ "params"] : the parameters for bcftools filter. An example is provided
11
+ * vep:
12
+ 1 ) cache_dir: directory in which the VEP cache should be downloaded
13
+ 2 ) zip_name: name of the zip file to download
14
+ 3 ) url: complete URL of the file to download
15
+ 4 ) image: docker image on which to create a container with Docker or Singularity
16
+ 5 ) filters: "--filter "
17
+ 6 ) impact_levels: select the impact level, as a list of strings (ex. [ "MODERATE", "HIGH"]
18
+ 7 ) species: currently only needed to give a directory name
You can’t perform that action at this time.
0 commit comments